Thursday, June 27, 2013

Cloning mice: For the first time, a donor mouse has been cloned using a drop of peripheral blood from its tail

June 26, 2013 ? From obesity to substance abuse, from anxiety to cancer, genetically modified mice are used extensively in research as models of human disease. Researchers often spend years developing a strain of mouse with the exact genetic mutations necessary to model a particular human disorder. But what if that mouse, due to the mutations themselves or a simple twist of fate, was infertile?

Currently, two methods exist for perpetuating a valuable strain of mouse. If at least one of the remaining mice is male and possesses healthy germ cells, the best option is intracytoplasmic sperm injection (ICSI), an in vitro fertilization procedure in which a single sperm is injected directly into an egg.

However, if the remaining mice cannot produce healthy germ cells, or if they are female, researchers must turn to cloning. Somatic-cell nuclear transfer (SCNT) produces cloned animals by replacing an oocyte's nucleus with that of an adult somatic cell. An early version of this process was used to produce Dolly the sheep in 1996.

Since then, SCNT techniques have continued to advance. Earlier this year, researchers at the RIKEN Center for Developmental Biology in Kobe, Japan, even devised a technique to avoid the diminishing returns of recloning the same cell; success rates increased from the standard three percent in first-generation clones to ten percent in first-generation and 14 percent in higher-generation clones.

The type of somatic cell used for this process is critical and depends largely on its efficiency in producing live clones, as well as its ease of access and readiness for experimental use. While cumulus cells, which surround oocytes in the ovarian follicle and after ovulation, are currently the preferred cell type, Drs. Satoshi Kamimura, Atsuo Ogura, and colleagues at the RIKEN BioResource Center in Tsukuba, Japan, questioned whether white blood cells (a.k.a., leukocytes) collected from an easily accessed site, such as a tail, would be effective donor cells. Such cells would allow for repeated sampling with minimal risk to the donor mouse.

There are five different types of white blood cells and, as expected, the researchers found that lymphocytes were the type that performed the most poorly: only 1.7 percent of embryos developed into offspring. The physically largest white blood cells, and thus the easiest to filter from the blood sample, were granulocytes and monocytes. The nuclei of these cells performed better, with 2.1 percent of the embryos surviving to term, compared to 2.7 percent for the preferred cell type, cumulus cells.

The granulocytes' performance was poorer than expected due to a much higher rate of fragmentation in early embryos (22.6 percent): twofold higher than that of lymphocyte cloning and fivefold higher than cumulus cell cloning. The researchers were unable to determine what could be causing the fragmentation and intend to perform further studies to improve the performance of granulocyte donor cells.

Although the blood cells tested did not surpass the success rate of cumulus cells in this study, the researchers have demonstrated, for the first time, that mice can be cloned using the nuclei of peripheral blood cells. These cells may be used for cloning immediately after collection with minimal risk to the donor, helping to generate genetic copies of mouse strains that cannot be preserved by other assisted reproduction techniques.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/~3/GDH_lb66SVk/130626153920.htm

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Past brain activation revealed in scans: Brain activity patterns preserve traces of previous cognitive activity

June 25, 2013 ? What if experts could dig into the brain, like archaeologists, and uncover the history of past experiences? This ability might reveal what makes each of us a unique individual, and it could enable the objective diagnosis of a wide range of neuropsychological diseases. New research at the Weizmann Institute hints that such a scenario is within the realm of possibility: It shows that spontaneous waves of neuronal activity in the brain bear the imprints of earlier events for at least 24 hours after the experience has taken place.

The new research stems from earlier findings in the lab of Prof. Rafi Malach of the Institute's Neurobiology Department and others that the brain never rests, even when its owner is resting. When a person is resting with closed eyes -- that is, no visual stimulus is entering the brain -- the normal bursts of nerve cell activity associated with incoming information are replaced by ultra-slow patterns of neuronal activity. Such spontaneous or "resting" waves travel in a highly organized and reproducible manner through the brain's outer layer -- the cortex -- and the patterns they create are complex, yet periodic and symmetrical.

Like hieroglyphics, it seemed that these patterns might have some meaning, and research student Tal Harmelech, under the guidance of Malach and Dr. Son Preminger, set out to uncover their significance. Their idea was that the patterns of resting brain waves may constitute "archives" for earlier experiences. As we add new experiences, the activation of our brain's networks lead to long-term changes in the links between brain cells, a facility referred to as plasticity. As our experiences become embedded in these connections, they create "expectations" that come into play before we perform any type of mental task, enabling us to anticipate the result. The researchers hypothesized that information about earlier experiences would thus be incorporated into the links between networks of nerve cells in the cortex, and these would show up in the brain's spontaneously emerging wave patterns.

In the experiment, the researchers had volunteers undertake a training exercise that would strongly activate a well-defined network of nerve cells in the frontal lobes. While undergoing scans of their brain activity in the Institute's functional magnetic resonance imaging (fMRI) scanner, the subjects were asked to imagine a situation in which they had to make rapid decisions. The subjects received auditory feedback in real time, based on the information obtained directly from their frontal lobe, which indicated the level of neuronal activity in the trained network. This "neurofeedback" strategy proved highly successful in activating the frontal network -- a part of the brain that is notoriously difficult to activate under controlled conditions.

To test whether the connections created in the brain during this exercise would leave their traces in the patterns formed by the resting brain waves, the researchers performed fMRI scans on the resting subjects before the exercise, immediately afterward, and 24 hours later. Their findings, which appeared in the Journal of Neuroscience, showed that the activation of the specific areas in the cortex did indeed remodel the resting brain wave patterns. Surprisingly, the new patterns not only remained the next day, they were significantly strengthened. These observations fit in with the classic learning principles proposed by Donald Hebb in the mid-20th century, in which the co-activation of two linked nerve cells leads to long term strengthening of their link, while activity that is not coordinated weakens this link. The fMRI images of the resting brain waves showed that brain areas that were activated together during the training sessions exhibited an increase in their functional link a day after the training, while those areas that were deactivated by the training showed a weakened functional connectivity.

This research suggests a number of future possibilities for exploring the brain. For example, spontaneously emerging brain patterns could be used as a "mapping tool" for unearthing cognitive events from an individual's recent past. Or, on a wider scale, each person's unique spontaneously emerging activity patterns might eventually reveal a sort of personal profile -- highlighting each individual's abilities, shortcomings, biases, learning skills, etc. "Today, we are discovering more and more of the common principles of brain activity, but we have not been able to account for the differences between individuals," says Malach. "In the future, spontaneous brain patterns could be the key to obtaining unbiased individual profiles." Such profiles could be especially useful in diagnosing or learning the brain pathologies associated with a wide array of cognitive disabilities.

Prof. Rafi Malach's research is supported by the Nella and Leon Benoziyo Center for Neurosciences; the Nella and Leon Benoziyo Center for Neurological Diseases; the Carl and Micaela Einhorn-Dominic Brain Research Institute; the Norman and Helen Asher Center for Human Brain Imaging; the Murray H. and Meyer Grodetsky Center for Research of Higher Brain Functions; the Kahn Family Research Center for Systems Biology of the Human Cell; the Friends of Dr. Lou Siminovitch; the Adelis Foundation; and the Mike and Valeria Rosenbloom through the Mike Rosenbloom Foundation. Prof. Malach is the recipient of the Helen and Martin Kimmel Award for Innovative Investigation; and he is the incumbent of the Barbara and Morris L. Levinson Professorial Chair in Brain Research.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/strange_science/~3/ACKTq5RWxG8/130625121153.htm

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Farrah Abraham Completes Alcohol Education Course

Source: http://www.thehollywoodgossip.com/2013/06/farrah-abraham-completes-alcohol-education-course/

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Wednesday, June 26, 2013

3 Habitable-Zone Super-Earths Found Orbiting Nearby Star

Yeah, but 11 years ago getting from NY to London in less than 4 hours was an everyday thing (if pricier than other flights). Now it's unheard of.

Yes but it was sort of like the pony express shutting down their rush service because the telegraph arrived, maybe that sucks if you wanted to send a package but for the 95% that wanted to send a letter the telegraph was faster and better. Not every aspect of every old service is going to be preserved by the new ones, there will always be some regressions in the overall picture. Even though we're making incremental improvements I doubt we'll see any revolutionary changes in things like jet propulsion, internal combustion, gas turbines and whatnot - it's just minor tweaks to squeeze more efficiency out of it.

The overwhelming number of changes I expect is for things to get smaller, smarter and for more and more things to go electronically rather than physically and applying brute force. Maybe you get another 5 mph on the interstate but the main difference is an AI that drives itself. My dream of "real technological development" would be things like having nanobots to destroy bacteria, viruses, toxins, cancer cells, cure genetic diseases and prevent aging on the cell level. In the future maybe we all have personal assistants like only the rich have today, only they're robotic. It couldn't be done today because to have servants somebody would have to be the servants, but we could all have a robot the way we all have cell phones.

I'm not going to bash the system we have today, I can go down to the grocery store and buy a finished meal, pop it in the microwave and put the dishes in the dishwasher but it certainly could be taken to the next level where I just tell a robot I'd like spaghetti bolognese today and it'd shop, cook like a professional chef, serve and clear the tables when I'm done. Having a washing machine and a dryer is also rather relaxed, but again being able to throw dirty clothes in the bin and have them sorted, washed, dried, ironed if applicable and put back in the closest by themselves would be even better. Roombas and electronic lawn mowers are just a shadow of what robot housekeepers and gardeners could be. In short, even if I don't see flying cars on the horizon I see plenty things that could make life in 2013 seem rather primitive compared to 100 years from now.

Source: http://rss.slashdot.org/~r/Slashdot/slashdotScience/~3/tUER4nHQy2g/story01.htm

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Samsung denies giving up on desktop PC tower business

Samsung 'will continue to open all possibilities in PC business,' including PC towers

Yesterday, The Korea Times reported that Samsung was to close its "unprofitable" desktop PC business as "demand for conventional desktop PCs is going down," according to an unnamed spokesperson. The weirdly written article also quoted another Samsung official saying "tablets, all-in-one and hybrid PCs are Samsung's current focus," thus suggesting the company seemed to be singling out desktop PC towers or boxes.

Even though it's been a while since some of us last saw a Samsung desktop PC tower (the latest models we covered date back to 2006, though there have been more recent efforts), something didn't smell right here so we reached out to Samsung directly. The response we got was that this rumor is all "groundless," and the company also specifically said it'll keep an open mind about its PC tower business. Here's the full statement:

"The rumor that Samsung is withdrawing from the PC desktop business is groundless. Samsung will continue to offer diverse products according to market needs, including our recently announced ATIV One 5 Style, a stylish all-in-one PC. We will continue to open all possibilities in PC business including our PC Tower business, to satisfy consumer's diverse lifestyle and needs."

So in a nutshell: nothing to see here, move along. And technically speaking, the Chromebox kinda counts too, right?

Update: Samsung just informed us that it actually launched a couple of desktop PC towers, the 700T3A and 300T3A, in January this year, but only for the Korean B2C market. We got them pictured after the break.

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Source: http://www.engadget.com/2013/06/25/samsung-desktop-pc-tower-business/?utm_medium=feed&utm_source=Feed_Classic&utm_campaign=Engadget

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Can An Old Massachusetts Fishing Port Light The World Again?

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Obama aims to tackle pollution, climate change

President Barack Obama wipes his face as he speaks about climate change, Tuesday, June 25, 2013, at Georgetown University in Washington. The president is proposing sweeping steps to limit heat-trapping pollution from coal-fired power plants and to boost renewable energy production on federal property, resorting to his executive powers to tackle climate change and sidestepping the partisan gridlock in Congress. (AP Photo/Charles Dharapak)

President Barack Obama wipes his face as he speaks about climate change, Tuesday, June 25, 2013, at Georgetown University in Washington. The president is proposing sweeping steps to limit heat-trapping pollution from coal-fired power plants and to boost renewable energy production on federal property, resorting to his executive powers to tackle climate change and sidestepping the partisan gridlock in Congress. (AP Photo/Charles Dharapak)

President Barack Obama speaks about climate change, Tuesday, June 25, 2013, at Georgetown University in Washington. The president is proposing sweeping steps to limit heat-trapping pollution from coal-fired power plants and to boost renewable energy production on federal property, resorting to his executive powers to tackle climate change and sidestepping the partisan gridlock in Congress. (AP Photo/Charles Dharapak)

(AP) ? President Barack Obama declared the debate over climate change and its causes obsolete Tuesday as he announced a wide-ranging plan to tackle pollution and prepare communities for global warming.

In a major speech at Georgetown University, Obama warned Americans of the deep and disastrous effects of climate change, urging them to take action before it's too late.

"As a president, as a father and as an American, I'm here to say we need to act," Obama said.

Obama announced he was directing his administration to launch the first-ever federal regulations on heat-trapping gases emitted by new and existing power plants ? "to put an end to the limitless dumping of carbon pollution."

Other aspects of the plan will boost renewable energy production on federal lands, increase efficiency standards and prepare communities to deal with higher temperatures.

Even before Obama unveiled his plan Tuesday, Republican critics in Congress were lambasting it as a job-killer that would threaten the economic recovery. Obama dismissed those critics, noting the same arguments have been used in the past when the U.S. has taken other steps to protect the environment.

"That's what they said every time," Obama said. "And every time, they've been wrong."

Obama also offered a rare insight into his administration's deliberations on Keystone XL, an oil pipeline whose potential approval has sparked an intense fight between environmental activists and energy producers.

The White House has insisted the State Department is making the decision independently, but Obama said Tuesday he's instructing the department to approve it only if the project won't increase overall, net emissions of greenhouse gases.

"Allowing the Keystone pipeline to be built requires a finding that doing so would be in our nation's interests," Obama said. "Our national interest would be served only if this project does not significantly exacerbate the problem of carbon pollution."

A top aide to House Speaker John Boehner said the remarks indicated that the pipeline should be approved.

"The standard the president set today should lead to speedy approval of the Keystone pipeline," Boehner spokesman Brendan Buck said.

Still, environmentalist took heart in Obama's remarks, noting it was the first time the administration had directly linked approval of the pipeline to its effect on pollution. The White House has previously resisted efforts by environmental groups to link the Keystone project to broader effort curb carbon pollution from power plants.

Obama touted America's strengths ? research, technology and innovation ? as factors that make the U.S. uniquely poised to take on the challenges of global warming. He mocked those who deny that humans are contributing to the warming of the planet, adding that he "doesn't have much patience" for anybody who refuses to acknowledge the problem.

"We don't have time for a meeting of the flat-earth society," Obama said.

Obama's far-reaching plan marks the president's most prominent effort yet to deliver on a major priority he laid out in his first presidential campaign and recommitted to at the start of his second term: to fight climate change in the U.S. and abroad and prepare American communities for its effects. Environmental activists have been irked that Obama's high-minded goals never materialized into a comprehensive plan.

By expanding permitting on public lands, Obama hopes to generate enough electricity from renewable energy projects such as wind and solar to power the equivalent of 6 million homes by 2020, effectively doubling the electric capacity federal lands now produce. He also set a goal to install 100 megawatts of energy-producing capacity at federal housing projects by the end of the decade.

Obama also announced $8 billion in federal loan guarantees to spur investment in technologies that can keep carbon dioxide produced by power plants from being released into the atmosphere.

But the linchpin of Obama's plan is the controls on new and existing power plants. Forty percent of U.S. carbon dioxide emissions, and one-third of greenhouse gases overall, come from electric power plants, according to the federal Energy Information Administration. The Obama administration already has proposed controls on new plants, but those controls have been delayed and not yet finalized.

Tuesday's announcement came just weeks after Obama's nominee to head the Environmental Protection Agency, Gina McCarthy, assured senators during her confirmation process that the EPA was "not currently" developing any regulations on existing sources of greenhouse gases. McCarthy said if EPA were to look at such regulations, it would allow states, the public and others to "offer meaningful input on potential approaches."

Republicans quickly dismissed Obama's plan, calling it a "war on coal" and a "war on jobs," reflecting the opposition to climate legislation on Capitol Hill that prompted a frustrated Obama to sidestep lawmakers and take action himself.

"It's tantamount to kicking the ladder out from beneath the feet of many Americans struggling in today's economy," Senate Minority Leader Mitch McConnell, R-Ky., said on the floor of the Senate.

Environmental groups offered a mix of praise and wariness that Obama would follow through on the ambitious goals he laid out. Bill Snape of the Center for Biological Diversity described it as too little, too late.

"What he's proposing isn't big enough, doesn't move fast enough, to match the terrifying magnitude of the climate crisis," Snape said.

Others hailed the plan, galvanized by the fact that Obama was taking action on his own after Congress' reluctance to tackle the issue using legislation.

"The president nailed it: this can't wait," said Frances Beinecke, president of the Natural Resources Defense Council. "We will cut this carbon pollution today so our children don't inherit climate chaos tomorrow."

___

Follow Matthew Daly on Twitter: https://twitter.com/MatthewDalyWDC

Follow Josh Lederman on Twitter: https://twitter.com/joshledermanAP

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/89ae8247abe8493fae24405546e9a1aa/Article_2013-06-25-US-Obama-Climate-Change/id-e98675d6ef804809b3a03bd125ad17ec

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